兔 anti-大鼠 IgA, IgG, IgM Antibody (HRP) - Preadsorbed
ELISA, IHC, ICC
Polyclonal
IgG
HRP
产品编号 ABIN458728
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 兔 anti-大鼠 IgA, IgG, IgM Antibody (HRP) - Preadsorbed (ABIN458728)
抗原
IgA, IgG, IgM
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
亚型
IgG
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适用
- 大鼠
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宿主
- 兔
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特异性
- The reactivity of the antiserum is directed to the major isotypes of the rat immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.
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产品特性
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Horseradish peroxidase-conjugated IgG fraction of polyclonal rabbit antiserum to rat IgG, IgA and IgM, heavy and light chains
Peroxidase/IgG protein molar ratio (E/P) is approximately 1.3. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2). -
纯化方法
- Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
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免疫原
- Purified polyclonal rat IgG, and homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
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In enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. The presence of activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents or GARa/Ig (Fc) /PO should be used instead for this purpose. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:10,000. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
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浓度
- 10 mg/mL
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缓冲液
- Peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
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储存液
- Without preservative
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注意事项
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Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. -
储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgA, IgG, IgM
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别名
- IgG + IgA + IgM
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物质类
- Antibody
抗原
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