兔 anti-大鼠 IgG2ab, Subclass Specific Antibody
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北京 101111
Quick Overview for 兔 anti-大鼠 IgG2ab, Subclass Specific Antibody (ABIN458718)
抗原
克隆类型
应用范围
亚型
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适用
- 大鼠
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宿主
- 兔
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特异性
- The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b.
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产品特性
- Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. Antibody titre: Precipitin titre not less then 1:64 when tested against pooled normal rat serum in agar block immunodiff
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纯化方法
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Delipidated, heat inactivated, lyophilized whole antiserum.
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. -
免疫原
- Pools of purified homogenous IgG2a and IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2a and/or IgG2b in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG2a/IgG2b from serum or plasma.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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缓冲液
- No foreign proteins added.
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储存液
- Without preservative
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注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgG2ab (IgG2ab, Subclass Specific)
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别名
- IgG2ab
抗原
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