兔 anti-大鼠 IgA, IgG, IgM (Heavy & Light Chain) Antibody
IEP, RID
Polyclonal
IgG
unconjugated
产品编号 ABIN458715
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 兔 anti-大鼠 IgA, IgG, IgM (Heavy & Light Chain) Antibody (ABIN458715)
抗原
IgA, IgG, IgM
克隆类型
多克隆
应用范围
Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
亚型
IgG
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抗原表位
- Heavy & Light Chain
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适用
- 大鼠
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宿主
- 兔
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原理
- Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains)
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特异性
- Precipitating polyclonal Rabbit antiSerum to Rat immunoglobulins, IgG, IgA and IgM, heavy and light chains.
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- The reactivity of the antiserum is directed to the major isotypes of the rat immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal rat serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
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纯化方法
- Delipidated, heat inactivated lyophilized stable whole serum
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免疫原
- Purified polyclonal IgG, including all subclasses and pools of homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- Precipitation assays. In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated, lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit s
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- IgA, IgG, IgM
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别名
- IgG + IgA + IgM
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物质类
- Antibody
抗原
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