山羊 anti-大鼠 IgG2c (Subclass specific) Antibody (Biotin) - Preadsorbed
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北京 101111
Quick Overview for 山羊 anti-大鼠 IgG2c (Subclass specific) Antibody (Biotin) - Preadsorbed (ABIN458702)
抗原
克隆类型
标记
应用范围
亚型
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抗原表位
- Subclass specific
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适用
- 大鼠
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宿主
- 山羊
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原理
- Goat anti Rat IgG2c (subclass specific), conjugated with Biotin
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特异性
- Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Rat IgG2c, subclass specific.
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交叉反应 （详细）
- This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this immunoconjugate has not been tested in detail.
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产品特性
- The reactivity of the antiserum is directed to the subclass IgG2c. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgG2c at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2c antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of rat origin known to be of the IgG2c isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2c in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000 depending on the method used.
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纯化方法
- Purified
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免疫原
- Pools of purified homogenous IgG2c isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Mouse anti-Rat IgG2c antibody
ELISA, FACS, IHC (fro) Monoclonal 2C8F1 IgG2a unconjugated
Mouse anti-Rat IgG2c antibody (HRP)ELISA, IHC (fro) Monoclonal 2C8F1 IgG2a HRP
Mouse anti-Rat IgG2c antibody (Alkaline Phosphatase (AP))ELISA, IHC (fro) Monoclonal 2C8F1 IgG2a Alkaline Phosphatase (AP)
Mouse anti-Rat IgG2c antibody (Biotin)ELISA, FACS, IHC (fro) Monoclonal 2C8F1 IgG2a Biotin
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- IgG2c
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物质类
- Antibody
抗原
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