山羊 anti-Mouse IgA, IgG, IgM (Heavy & Light Chain) Antibody (HRP) - Preadsorbed
ELISA, WB, ICC, DB, IHC (p)
Polyclonal
IgG
HRP
产品编号 ABIN458683
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 山羊 anti-Mouse IgA, IgG, IgM (Heavy & Light Chain) Antibody (HRP) - Preadsorbed (ABIN458683)
抗原
IgA, IgG, IgM
克隆类型
多克隆
标记
HRP
应用范围
ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
亚型
IgG
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抗原表位
- Heavy & Light Chain
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适用
- 人, 小鼠, 大鼠
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宿主
- 山羊
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原理
- Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains) (non Human and Mouse), conjugated with Horseradish peroxidase
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特异性
- Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Rat IgG, IgA and IgM, heavy and light chains, adsorbed for Human cross-reactivity.
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. If the product is to be used in the presence of material originating from an other species than Human and Mouse, prior screening for cross-reactivity with that material is essential and additional adsorption of the reagent may be required to ensure intra-assay specificity.
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产品特性
- he reactivity of the antiserum is directed to the major isotypes of the rat immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In enzyme-immunocytochemical and immunohistochemical staining of monoclonal rat monoclonal antibodies in the presence of human cell or serum components at the cellular and subcellular level. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Purified polyclonal rat IgG, and homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Goat anti-Human IgA, IgG, IgM antibody (HRP)
ELISA, IHC, WB, IP Polyclonal IgG HRP
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- IgA, IgG, IgM
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别名
- IgG + IgA + IgM
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物质类
- Antibody
抗原
-
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