猪 anti-兔 IgG (Heavy & Light Chain) Antibody
IP
Polyclonal
unconjugated
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 兔
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宿主
- 猪
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克隆类型
- 多克隆
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应用范围
- Immunoprecipitation (IP)
- 特异性
- Precipitating polyclonal Swine antiSerum to Rabbit IgG, heavy and light chains.
- 交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
- 产品特性
- The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal human serum, as bridging reagent in a peroxidase antiperoxidase technique using R/PAP, to prepare an immunoadsorbent for the production of Ig-free rabbit serum, plasma or non-Ig preparations
- 纯化方法
- Purified
- 免疫原
- Purified normal IgG isolated from pooled rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Goat anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, FM, IP, LF Polyclonal IgG unconjugated
Guinea Pig anti-Rabbit IgG antibody (HRP)ELISA, IHC, WB, IP Polyclonal IgG HRP
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- 应用备注
- In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water.Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. It is reconstituted by adding 1 mL sterile distilled water.
- 缓冲液
- Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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