绵羊 anti-小鼠 IgG2a (Allotype 1b) Antibody
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北京 101111
Quick Overview for 绵羊 anti-小鼠 IgG2a (Allotype 1b) Antibody (ABIN458534)
抗原
克隆类型
应用范围
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抗原表位
- Allotype 1b
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适用
- 小鼠
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宿主
- 绵羊
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原理
- Sheep anti Mouse IgG2a (1b allotype specific)
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特异性
- Purified IgG fraction of polyclonal Sheep antiSerum to Mouse IgG2a(1b), allotype specific.
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交叉反应 （详细）
- The antiSerum does not cross-react with any other component of the Mouse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, expected chance of cross-reactivity will be small.
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产品特性
- The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1b, including C57Bl and SJL/J, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1b). No precipitation reaction is obtained with purified IgG2a(1a) (Balb/C, CBA, AKR and NZB), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. As unlabelled primary or secondary reagent for indirect detection of IgG2a(1b) at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Highly purified normal homogenous IgG2a(1b) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Goat anti-Mouse IgG2a antibody (HRP) - Preadsorbed
ELISA, WB, ICC, IHC (fro), IHC (p), ELISpot Polyclonal IgG HRP
Rat anti-Mouse IgG2a antibody (HRP)ELISA, WB, ICC, IHC (fro) Monoclonal SB84a IgG1 HRP
Rat anti-Mouse IgG2a antibodyELISA, FACS, WB, ICC, IHC (fro), MA Monoclonal SB84a IgG1 unconjugated
Goat anti-Mouse IgG2a antibody (Biotin) - PreadsorbedELISA, FACS, IHC (fro) Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Mouse IgG2a antibody - PreadsorbedELISA, FACS, IHC (fro) Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Mouse IgG2a antibody (Biotin) - PreadsorbedELISA, FACS, WB, ICC, IHC (fro), IHC (p), ELISpot Polyclonal IgG Biotin
Goat anti-Mouse IgG2a antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, WB, ICC, IHC (fro), IHC (p), ELISpot Polyclonal IgG Alkaline Phosphatase (AP)
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应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Purified hyperimmune IgG fraction, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- IgG2a
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物质类
- Antibody
抗原
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