兔 anti-小鼠 IgG (Fab Region) Antibody - Preadsorbed
Our Local Distributor
北京 101111
Quick Overview for 兔 anti-小鼠 IgG (Fab Region) Antibody - Preadsorbed (ABIN458523)
抗原
克隆类型
应用范围
亚型
-
-
抗原表位
- Fab Region
-
适用
- 小鼠
-
宿主
- 兔
-
原理
- Rabbit anti Mouse IgG Fab
-
特异性
- Purified IgG fraction of polyclonal Rabbit antiSerum to Mouse Fab of IgG
-
交叉反应
- Cow, Hamster, Pig, 大鼠
-
无交叉反应
- Cat, 小鸡, 犬, 鸭, 马, 人, 猴, 绵羊, Turkey
-
交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion with the following results: Bovine + Dog - Guinea Pig + Human - Rat + Turkey - Cat - Duck - Hamster + Monkey - Sheep - Chicken - Goat ± Horse - pigeon - Swine + A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
-
产品特性
- The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. For indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
-
纯化方法
- Purified IgG fraction from antiserum
-
免疫原
- Purified Fab of normal mouse IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
-
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - Preadsorbed
FACS, FLISA, FM, DB Polyclonal IgG TRITC
Goat anti-Mouse IgG antibody (DyLight 800)WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Rabbit anti-Mouse IgG antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment
Donkey anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488) - PreadsorbedIHC, WB, FACS, IF, ICC Polyclonal IgG DyLight 488
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488) - PreadsorbedIHC, WB, FACS, IF, ICC Polyclonal IgG DyLight 488
-
-
应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
-
缓冲液
- Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
-
-
- IgG
-
物质类
- Antibody
抗原
-
