绵羊 anti-小鼠 IgG2a(1a) (Fc Region) Antibody (FITC) - Preadsorbed
ICC, ELISA, WB, IHC
Polyclonal
IgG
FITC
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- 抗原
- IgG2a(1a)
- 抗原表位
- Fc Region
- 适用
- 小鼠
-
宿主
- 绵羊
-
克隆类型
- 多克隆
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标记
- FITC
- 应用范围
- Immunocytochemistry (ICC), ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1a, including Balb/C, CBA AKR and NZB, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1a). No precipitation reaction is obtained with purified IgG2a(1b) (C57Bl and SJL/J), other IgG subclasses, IgA, IgM, IgG/Fab fragments or other serum proteins
- 产品特性
-
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal sheep antiserum to mouse IgG2a(1a), allotype specific
Fluorochrome/ IgG molar ratio is approximately 1.6. - 纯化方法
- Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
- 免疫原
- Highly purified normal homogenous IgG2a(1a) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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-
- 应用备注
- In immunocytochemical and immunohistochemical staining for the detection of IgG2a (1a) at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA and Western blotting) to identify and measure IgG2a (1a) in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. Its optimum working dilution should be established by titration. Excess labelled antibody may by itself cause high unspecific background staining or interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40. These data should be interpreted as general recommendations only.
- 说明
-
Excitation/Emission wavelength: 494 nm/514 nm
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
- 浓度
- 10 mg/mL
- 缓冲液
- Fluorochrome-coupled hyperimmune IgG fraction, lyophilized from a solution in phosphate buffered saline (pH 7.2).
- 储存液
- Without preservative
- 注意事项
- Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- 抗原
- IgG2a(1a)
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