绵羊 anti-小鼠 IgG2a(1a) (Allotype 1a) Antibody
ID, IEP, ICC
Polyclonal
IgG
unconjugated
产品编号 ABIN458480
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 绵羊 anti-小鼠 IgG2a(1a) (Allotype 1a) Antibody (ABIN458480)
抗原
IgG2a(1a)
克隆类型
多克隆
应用范围
Immunodiffusion (ID), Immunoelectrophoresis (IEP), Immunocytochemistry (ICC)
亚型
IgG
-
-
抗原表位
- Allotype 1a
-
适用
- 小鼠
-
宿主
- 绵羊
-
原理
- Sheep anti Mouse IgG2a (1a allotype specific)
-
特异性
- Precipitating polyclonal Sheep antiSerum to Mouse IgG2a(1a), allotype specific.
-
交叉反应 （详细）
- The antiSerum does not cross-react with any other component of the Mouse Ig system. Inter-species cross reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, expected chance of cross-reactivity will be small.
-
产品特性
- The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1a, including Balb/C, CBA, AKR and NZB, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1a). No precipitation reaction is obtained with purified IgG2a(1b) (C57Bl and SJL/J), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2a(1a) in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgG2a(1a) from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. For such use labelled an unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity an performance requirements are available.
-
纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
-
免疫原
- Highly purified normal homogenous IgG2a(1a) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
-
-
-
应用备注
- Precipitation assays.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
-
缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added.
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
-
-
- IgG2a(1a)
抗原
-
You are here:
