绵羊 anti-小鼠 IgG (Fab Region) Antibody
IEP, ODD
Polyclonal
IgG
unconjugated
产品编号 ABIN458478
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 绵羊 anti-小鼠 IgG (Fab Region) Antibody (ABIN458478)
抗原
IgG
克隆类型
多克隆
应用范围
Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)
亚型
IgG
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抗原表位
- Fab Region
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适用
- 小鼠
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宿主
- 绵羊
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原理
- Sheep anti Mouse IgG Fab
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特异性
- Precipitating polyclonal Sheep antiSerum to Mouse Fab of IgG.
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been not tested in detail.
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产品特性
- The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of Fab, free or bound.
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纯化方法
- Delipidated, heat inactivated lyophilized stable whole serum
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免疫原
- Purified Fab of normal mouse IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- Precipitation assays. In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated lyophilized stable whole serum No preservative added. Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. No foreign proteins added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- IgG
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物质类
- Antibody
抗原
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