兔 anti-小鼠 IgG2b Antibody
ID, IEP, RID
Polyclonal
IgG
unconjugated
产品编号 ABIN458439
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 兔 anti-小鼠 IgG2b Antibody (ABIN458439)
抗原
IgG2b
克隆类型
多克隆
应用范围
Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
亚型
IgG
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适用
- 小鼠
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宿主
- 兔
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特异性
- The reactivity of the antiserum is directed to the subclass IgG2b.
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产品特性
- Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. Antibody titre: Precipitin titre not les than 1:32 when tested against pooled normal mouse serum in agar block immunodif
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纯化方法
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Delipidated, heat inactivated, lyophilized, stable whole antiserum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis. -
免疫原
- Pools of purified homogenous IgG2b isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- In immunoelectrophoresis use 2 µL or equivalent against 120 µL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µL antiserum in a 3 mm diameter centre well and 2 µL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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缓冲液
- No foreign proteins added.
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储存液
- Without preservative
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注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not ref rozen, and preferably used the same day.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgG2b
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物质类
- Antibody
抗原
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