兔 anti-小鼠 IgG1, IgG2a, IgG2b, IgG3 (Fc Region) Antibody
IP
Polyclonal
unconjugated
Quick Overview for 兔 anti-小鼠 IgG1, IgG2a, IgG2b, IgG3 (Fc Region) Antibody (ABIN458430)
抗原
IgG1, IgG2a, IgG2b, IgG3
克隆类型
多克隆
应用范围
Immunoprecipitation (IP)
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抗原表位
- Fc Region
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适用
- 小鼠
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宿主
- 兔
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特异性
- Precipitating polyclonal Rabbit antiSerum to Mouse IgG, Fc specific
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion anti several sera with the following results: cow - Cat - Chicken - Dog - Duck - Goat - Guinea Pig+ Human - Hamster + Horse - Monkey - pigeon - Rat + Sheep - Swine - Turkey - The absence of a reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
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产品特性
- The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal mouse serum, to prepare an immunoadsorbent for the production of Ig-free mouse serum, plasma or non-Ig preparations.
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纯化方法
- Delipidated, heat inactivated, stable whole serum
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过滤
- Heat-inactivated
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免疫原
- Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water.Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added. It is reconstituted by adding 1 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum.
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储存液
- Without preservative
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储存条件
- RT,4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- IgG1, IgG2a, IgG2b, IgG3
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别名
- IgG1 IgG2a IgG2b IgG3
抗原
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