兔 anti-小鼠 IgG (Fab Region) Antibody
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北京 101111
Quick Overview for 兔 anti-小鼠 IgG (Fab Region) Antibody (ABIN458421)
抗原
克隆类型
应用范围
亚型
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抗原表位
- Fab Region
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适用
- 小鼠
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宿主
- 兔
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原理
- Rabbit anti Mouse IgG Fab
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特异性
- Precipitating polyclonal Rabbit antiSerum to Mouse Fab of IgG
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交叉反应
- Hamster, Pig, 大鼠
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无交叉反应
- Cat, 小鸡, 犬, 鸭, 马, 人, 猴, 绵羊, Turkey
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion with the following result: cow + Duck - Human - Rat + Cat - Goat ± Horse - Sheep - Chicken - Guinea Pig + Monkey - Swine + Dog - Hamster + pigeon - Turkey - A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
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产品特性
- The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of Fab, free or bound.
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纯化方法
- Delipidated, heat inactivated lyophilized stable whole serum
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免疫原
- Purified Fab of normal mouse IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - Preadsorbed
FACS, FLISA, FM, DB Polyclonal IgG TRITC
Goat anti-Mouse IgG antibody (DyLight 800)WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (PE) - PreadsorbedFACS, FLISA Polyclonal IgG PE F(ab')2 fragment
Rabbit anti-Mouse IgG antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment
Donkey anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488)IHC, FACS, IF, ICC Polyclonal IgG DyLight 488
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应用备注
- Precipitation assays.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins ad
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- IgG
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物质类
- Antibody
抗原
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