山羊 anti-小鼠 IgG2b Antibody
ID, IEP, RID
Polyclonal
IgG
unconjugated
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- 抗原 See all IgG2b products
- IgG2b
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
- 特异性
- The reactivity of the antiserum is directed to the subclass IgG2b.
- 产品特性
- Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. Antibody titre: Precipitin titre not les than 1:32 when tested against pooled normal mouse serum in agar block immunodiffu
- 纯化方法
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Delipidated, heat inactivated, lyophilized, stable whole antiserum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. - 免疫原
- Pools of purified homogenous IgG2b isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- In immunoelectrophoresis use 2 µL or equivalent against 120 µL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µL antiserum in a 3 mm diameter centre well and 2 µL serum samples (neat and diluted) in 2 mm diameter peripheral wells.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
- 缓冲液
- No foreign proteins added.
- 储存液
- Without preservative
- 注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not ref rozen, and preferably used the same day.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- 抗原
- IgG2b
- Abstract
- IgG2b 产品
- 物质类
- Antibody
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