山羊 anti-小鼠 IgA, IgG, IgM (Fc Region) Antibody
ICC, IEP
Polyclonal
IgG
unconjugated
产品编号 ABIN458344
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 山羊 anti-小鼠 IgA, IgG, IgM (Fc Region) Antibody (ABIN458344)
抗原
IgA, IgG, IgM
克隆类型
多克隆
应用范围
Immunocytochemistry (ICC), Immunoelectrophoresis (IEP)
亚型
IgG
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抗原表位
- Fc Region
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适用
- 小鼠
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宿主
- 山羊
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特异性
- The reactivity of the antiserum is directed to the Fc subunits of the major immunoglobulins.
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产品特性
- Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. Antibody titre: Precipitin titre not less than 1:128 when tested against normal mouse serum in agar block titration.
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纯化方法
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Delipidated, heat inactivated, lyophilized stable whole serum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. -
免疫原
- Purified polyclonal IgG and homogenous IgA and IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal mouse serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water.
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缓冲液
- No foreign proteins added.
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储存液
- Without preservative
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注意事项
- Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgA, IgG, IgM
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别名
- IgG + IgA + IgM
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物质类
- Antibody
抗原
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