山羊 anti-人 IgA, IgG, IgM Antibody - Preadsorbed
ELISA, WB, ICC
Polyclonal
IgG
unconjugated
产品编号 ABIN458211
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 山羊 anti-人 IgA, IgG, IgM Antibody - Preadsorbed (ABIN458211)
抗原
IgA, IgG, IgM
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
亚型
IgG
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适用
- 人
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宿主
- 山羊
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特异性
- The reactivity of the antiserum is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immuno-electrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.
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产品特性
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Purified IgG fraction of polyclonal goat antiserum to human immunoglobulins IgG, IgA, IgM, heavy and light chains
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纯化方法
- Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
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免疫原
- Highly purified IgG and pools of homogenous IgA and IgM isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Goat anti-Human IgA, IgG, IgM antibody (HRP)
ELISA, IHC, WB, IP Polyclonal IgG HRP
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应用备注
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The cytochemical grade of GAHu/Ig/7S allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted.
The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:5,000. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
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浓度
- 10 mg/mL
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缓冲液
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
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储存液
- Without preservative
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注意事项
- Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, pro longed storage at or below -24 °C.
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- IgA, IgG, IgM
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别名
- IgG + IgA + IgM
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物质类
- Antibody
抗原
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