山羊 anti-人 IgG (Fab Region) Antibody - Preadsorbed
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北京 101111
Quick Overview for 山羊 anti-人 IgG (Fab Region) Antibody - Preadsorbed (ABIN458209)
抗原
克隆类型
应用范围
亚型
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抗原表位
- Fab Region
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适用
- 人
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宿主
- 山羊
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原理
- Goat anti Human IgG Fab
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特异性
- Purified IgG fraction of polyclonal Goat antiSerum to Human Fab of IgG
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. For indirect staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa and lambda type. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Purified Fab from normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Mouse anti-Human IgG (Fc Region) antibody
Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Human IgG antibody (Biotin)ELISA, WB, FACS, IHC (fro), ELISpot, Sep Polyclonal IgG Biotin F(ab')2 fragment
Mouse anti-Human IgG (Domain CH2), (Fc Region) antibody (HRP)ELISA, IHC (fro) Monoclonal MK 1 A6 IgG1 HRP
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应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- IgG
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物质类
- Antibody
抗原
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