兔 anti-人 IgG3 (Subclass specific) Antibody
StM, CP, ODD, Ct
Polyclonal
IgG
unconjugated
产品编号 ABIN458150
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 兔 anti-人 IgG3 (Subclass specific) Antibody (ABIN458150)
抗原
IgG3
克隆类型
多克隆
应用范围
Staining Methods (StM), Control Peptide (CP), Ouchterlony Double Diffusion (ODD), Control (Ct)
亚型
IgG
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抗原表位
- Subclass specific
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适用
- 人
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宿主
- 兔
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原理
- Rabbit anti Human IgG3 (subclass specific)
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特异性
- Precipitating polyclonal Rabbit antiSerum to Human IgG3, subclass specific.
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交叉反应 （详细）
- The antiSerum does not cross-react with any other component of the Human immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- The reactivity of the antiserum is restricted to subclass specific determinants on IgG3. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal human serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG3. No precipitation reaction is obtained with purified other subclasses of IgG, IgA, IgM, and IgG/Fab fragments.
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纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
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免疫原
- Highly purified homogenous IgG3 isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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应用备注
- Precipitation assays. In double radial immunodiffusion (Ouchterlony) serum or plasma samples are prepared in serial two-fold dilutions from 1:8 to 1: 128 and placed in the peripheral wells of the rosette arrangement. One well is filled with pooled human serum or another control. The center wells are filled with the subclass specific antiserum and the sera are allowed to diffuse at ambient temperature for 16-24 hours. Frequently the precipitin lines become visible only after staining.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
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- IgG3
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物质类
- Antibody
抗原
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