兔 anti-人 kappa Light Chain (free) Antibody
IP
Polyclonal
unconjugated
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- 抗原 See all kappa Light Chain products
- kappa Light Chain
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抗原表位
- free
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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应用范围
- Immunoprecipitation (IP)
- 特异性
- Precipitating polyclonal Rabbit antiSerum to free Human Ig kappa chain.
- 交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
- 产品特性
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The reactivity of the antiserum is directed to the hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is only reacting with Bence Jones proteins as well as free light chains of the kappa type. No reaction is obtained with intact polyclonal or monoclonal immunoglobulins. To detect and identify free light chain type and Bence Jones protein in serum or urine by To detect and identify free light chain type and Bence Jones protein in serum or urine by immunoelectrophoresis or double radial immunodiffusion. It is of great importance as an aid in the diagnosis of B cell malignancies such a multiple myeloma and non-Hodgkin lymphoma, and in monitoring their therapy. In immunoelectrophoresis use 1.2 μL of sample in a well with a diameter of 1 mm against 60 μL antiserum in a 20 mm long channel. Electrophoresis for 1 hour, 20 mA. The detection limit of this technique in serum is about 25 mg/l, in the case 100 times concentrated urine is used the detection limit can be brought down to 0.5 mg/l. The fact that in about 40 % of the case the Bence Jones protein was only detectable in either serum or urine emphasizes the necessity to investigate both fluids.
Clone: Polyclonal - 纯化方法
- Delipidated, heat inactivated lyophilized whole serum.
- 过滤
- Heat-inactivated
- 免疫原
- A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.
- 缓冲液
- Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity.
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- kappa Light Chain
- 别名
- Ig kappa (kappa Light Chain 产品)
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