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兔 anti-人 IgM (Fc Region) Antibody

IP Polyclonal unconjugated
产品编号 ABIN458092
发货至: 中国

Quick Overview for 兔 anti-人 IgM (Fc Region) Antibody (ABIN458092)

抗原

IgM

克隆类型

  • 547
  • 102
多克隆

应用范围

  • 391
  • 296
  • 295
  • 256
  • 183
  • 145
  • 91
  • 91
  • 64
  • 62
  • 54
  • 53
  • 47
  • 37
  • 18
  • 17
  • 10
  • 6
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Immunoprecipitation (IP)
  • 抗原表位

    • 120
    • 86
    • 85
    • 20
    • 12
    • 10
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Fc Region

    适用

    • 231
    • 167
    • 92
    • 42
    • 34
    • 34
    • 30
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 13
    • 12
    • 4
    • 2
    • 2

    宿主

    • 373
    • 240
    • 91
    • 22
    • 14
    • 5
    • 4
    • 1
    • 1

    特异性

    Precipitating polyclonal Rabbit antiSerum to Human IgM, Fc specific.

    交叉反应 (详细)

    The antiSerum does not cross-react with any other component of the Human immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in immunoelectrophoresis a strong cross reaction with Serum of Rhesus Monkey, Cynomolgus and baboon has been observed.

    产品特性

    The reactivity of the antiserum is restricted to the Fc part of the IgG molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal human serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No precipitation reaction is obtained with purified IgA, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in human serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of human IgM from serum or plasma.

    纯化方法

    Delipidated, heat inactivated, stable whole serum

    过滤

    Heat-inactivated

    免疫原

    Highly purified normal IgM isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • 应用备注

    In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL in a 3 mm diameter center well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

    缓冲液

    Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.

    储存液

    Without preservative

    储存条件

    RT,4 °C,-20 °C

    储存方法

    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
  • 抗原

    IgM

    物质类

    Antibody
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