山羊 anti-人 IgA Antibody (HRP) - Preadsorbed
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北京 101111
Quick Overview for 山羊 anti-人 IgA Antibody (HRP) - Preadsorbed (ABIN458049)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 人
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宿主
- 山羊
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原理
- Goat anti Human J chain of dimeric IgA, conjugated with Horseradish peroxidase
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特异性
- Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Human J chain of dimeric IgA
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交叉反应 （详细）
- The antiSerum does not cross-react with any other component of Human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. J chains of many related specie (e.g. Mouse, Rabbit, Dog Cat Sheep and Swine) are known to posses a striking structural homology. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), this immunoconjugate shows a single precipitation reaction with totally reduced and alkylated polyclonal and monoclonal polymeric IgA, secretory IgA and IgM. A reaction of complete identity is obtained with precipitated highly purified J chain and with the single of precipitation obtained with normal human serum after two hours incubation with 9M urea and 0.2M mercaptoethanol at pH 8.6. In a competitive radioimmunoassay no inhibition is obtained with monomeric IgA, polyclonal IgG, reduced and alkylated alpha, mu, gamma, kappa and lambda light chains (less than 1 percent over background). In enzyme-immunocytochemical and immunohistochemical techniques for the detection of human J chain at the cellular and subcellular level in appropriately treated cell and tissue substrates, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Human J chain is a polypeptide folded within the structure of the polymeric immunoglobulin, resulting in a very limited exposure of J chain antigenic determinants. The antiserum is raised against the isolated and purified J chain. J chains isolated from polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity, Human J chain is distinct from all other chain components of polymeric IgA and IgM. It has a unique primary structure as shown by sequence analyses. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Rabbit anti-Human IgA (C-Term) antibody
ELISA, IHC, WB Polyclonal IgG unconjugated
Mouse anti-Human IgA (Heavy Chain) antibodyELISA, WB, IHC (fro), IP Monoclonal Mc24-2E11 IgG1 unconjugated
Rabbit anti-Human IgA (Chain alpha), (Heavy Chain) antibodyELISA, IHC, WB unconjugated
Mouse anti-Human IgA antibodyELISA, IHC (p) Monoclonal AD2-947 IgG1 unconjugated
Goat anti-Human IgA antibody (HRP)ELISA, WB, ICC, IHC (fro), IHC (p), ELISpot Polyclonal IgG HRP
Goat anti-Human IgA antibody (Biotin)ELISA, WB, FACS, ICC, IHC (fro), IP, IHC (p), ELISpot, MA Polyclonal IgG Biotin
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Horseradish peroxidase-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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- IgA
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物质类
- Antibody
抗原
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