山羊 anti-人 Ig lambda (Free Chain) Antibody
MA, EP, IEP, D
Polyclonal
unconjugated
产品编号 ABIN458045
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 山羊 anti-人 Ig lambda (Free Chain) Antibody (ABIN458045)
抗原
Ig lambda
克隆类型
多克隆
应用范围
Microarray (MA), Electrophoresis (EP), Immunoelectrophoresis (IEP), Detection (D)
-
-
抗原表位
- Free Chain
-
适用
- 人
-
宿主
- 山羊
-
原理
- Goat anti Human Ig lambda light chain (free)
-
特异性
- Precipitating polyclonal Goat antiSerum to free Human Ig lambda chain
-
交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
-
产品特性
- The reactivity of the antiserum is directed to the hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is only reacting with Bence Jones proteins as well as free light chains of the lambda type. No reaction is obtained with intact polyclonal or monoclonal immunoglobulins. To detect and identify free light chain type and Bence Jones protein in serum or urine by immunoelectrophoresis or double radial immunodiffusion. It is of great importance as an aid in the diagnosis of B cell malignancies such a multiple myeloma and non-Hodgkin lymphoma, and in monitoring their therapy.
-
纯化方法
- Delipidated, heat inactivated lyophilized stable whole serum
-
免疫原
- A pool of purified Bence Jones lambda proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
-
-
应用备注
- Precipitation assays. In immunoelectrophoresis use 1.2 μL of sample in a well with a diameter of 1 mm against 60 μL antiserum in a 20 mm long channel. Electrophoresis for 1 hour, 20 mA. The detection limit of this technique in serum is about 25 mg/l, in the case 100 times concentrated urine is used the detection limit can be brought down to 0.5 mg/l. The fact that in about 40 % of the case the Bence Jones protein was only detectable in either serum or urine emphasizes the necessity to investigate both fluids.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water and spin down to remove insoluble particles.
-
缓冲液
- Delipidated, heat inactivated and lyophilized stable whole antiserum. No preservative or foreign protein added. Total protein and IgG concentrations in the antiserum are comparable to those in pooled goat serum.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- The lyophilized product may be stored at +4°C, prolonged storage at or below -20°C. Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
-
-
- Ig lambda
抗原
-
You are here:
