小鼠 anti-人 IgG (Fc Region) Antibody
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北京 101111
Quick Overview for 小鼠 anti-人 IgG (Fc Region) Antibody (ABIN457927)
抗原
克隆类型
应用范围
克隆位点
亚型
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抗原表位
- Fc Region
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适用
- 人
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宿主
- 小鼠
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特异性
- The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. The antiserum reacts with the 4 subclasses of IgG, as tested in indirect binding enzyme immunoassay, immunoblotting and indirect immuno-peroxidase staining of cytoplasmic Ig. Monoclonal mouse antiserum to human IgG, isotype specific
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纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole ascites
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免疫原
- Highly purified IgG isolated from pooled human serum
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Mouse anti-Human IgG (Fc Region) antibody
Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgG (Heavy Chain) antibody (beta-Gal)ELISA, WB Polyclonal IgG beta-Gal
Goat anti-Human IgG (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)
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应用备注
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To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
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浓度
- 1.0 mg/mL
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缓冲液
- No foreign proteins added.
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注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgG
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物质类
- Antibody
抗原
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