小鼠 anti-人 IgA1 Antibody
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北京 101111
Quick Overview for 小鼠 anti-人 IgA1 Antibody (ABIN457922)
抗原
克隆类型
应用范围
克隆位点
亚型
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适用
- 人
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宿主
- 小鼠
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特异性
- The reactivity of the antiserum is restricted to a subclass specific determinant on the C H 2 domain of IgA1 as tested in haem- agglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Monoclonal mouse antiserum to human IgA1, subclass specific
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纯化方法
- Delipidated, heat inactivated, lyophilized, stable whole ascites
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免疫原
- Highly purified monoclonal IgA1 isolated from pooled human serum
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Mouse anti-Human IgA1 (Fc Region) antibody
ELISA Monoclonal B3506B4 IgG1 unconjugated
Mouse anti-Human IgA1 (Fc Region) antibody (HRP)WB, ELISA, IHC Monoclonal B3506B4 IgG1 HRP
Mouse anti-Human IgA1 antibody (PE)ELISA Monoclonal B3506B4 IgG1 PE
Mouse anti-Human IgA1 (Fc Region) antibody (Alkaline Phosphatase (AP))WB, ELISA, IHC Monoclonal B3506B4 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Human IgA1 (Fc Region) antibody (Biotin)ELISA Monoclonal B3506B4 IgG1 Biotin
Mouse anti-Human IgA1 antibody (FITC)ELISA Monoclonal B3506B4 IgG1 FITC
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应用备注
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To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgA1, haemagglutination and immunoblotting.
The optimum working dilu-tion is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:3000 to 1:50000. These data should be interpreted as general recommendations only. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
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浓度
- 1 mg/mL
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缓冲液
- No foreign proteins added.
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注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgA1
抗原
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