小鼠 anti-人 IgM Antibody (TRITC)
IF
Monoclonal
NI 179
IgG1 kappa
TRITC
Quick Overview for 小鼠 anti-人 IgM Antibody (TRITC) (ABIN457907)
抗原
IgM
克隆类型
单克隆
标记
TRITC
应用范围
Immunofluorescence (IF)
克隆位点
NI 179
亚型
IgG1 kappa
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适用
- 人
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宿主
- 小鼠
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特异性
- The reactivity of the antiserum is restricted to a isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunofluorescence staining of cytoplasmic Ig. Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
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产品特性
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Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
Fluorochrome/IgG protein molar ratio (E/P) approximately 5.7. -
纯化方法
- Purified
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免疫原
- Highly purified IgM isolated from human serum.
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应用备注
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To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).
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储存液
- Without preservative
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注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgM
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物质类
- Antibody
抗原
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