小鼠 anti-人 IgG2 Antibody (FITC)
Quick Overview for 小鼠 anti-人 IgG2 Antibody (FITC) (ABIN457889)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
-
-
适用
- 人
-
宿主
- 小鼠
-
特异性
- The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab part of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG2, subclass specific
-
产品特性
-
Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG2, subclass specific
Fluorochrome/IgG protein molar ratio (F/P) approximately 1.4. -
纯化方法
- Purified
-
免疫原
- Highly purified monoclonal IgG2 isolated from human serum.
-
-
Mouse anti-Human IgG2 (Fd Region) antibody
WB, ELISA, IHC, ICC Monoclonal HP6014 IgG1 unconjugated
Mouse anti-Human IgG2 (Fc Region) antibodyELISA Monoclonal HP6002 IgG1 unconjugated
Mouse anti-Human IgG2 (Fc Region) antibody (PE)ELISA Monoclonal HP6002 IgG1 PE
Mouse anti-Human IgG2 (Fd Region) antibody (Biotin)WB, ELISA, IHC, ICC Monoclonal HP6014 IgG1 Biotin
Mouse anti-Human IgG2 (Fc Region) antibody (Biotin)ELISA Monoclonal 31-7-4 IgG1 Biotin
Mouse anti-Human IgG2 (Fc Region) antibody (FITC)ELISA, FLISA Monoclonal 31-7-4 IgG1 FITC
Mouse anti-Human IgG2 (Fd Region) antibody (FITC)ELISA, IHC, ICC Monoclonal HP6014 IgG1 FITC
Mouse anti-Human IgG2 (Fc Region) antibody (HRP)WB, ELISA, IHC Monoclonal 31-7-4 IgG1 HRP
Mouse anti-Human IgG2 (Fc Region) antibody (Alkaline Phosphatase (AP))WB, ELISA, IHC Monoclonal 31-7-4 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Human IgG2 (Fd Region) antibody (PE)ELISA, IHC, ICC Monoclonal HP6014 IgG1 PE
-
-
应用备注
-
To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. -
说明
-
Excitation/Emission wavelength: 494 nm/514 nm
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
-
浓度
- 0.4 mg/mL
-
缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).
-
储存液
- Without preservative
-
注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
-
-
- IgG2
-
物质类
- Antibody
抗原
-
