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小鼠 anti-人 IgG2 Antibody (Biotin)

WB, ELISA Monoclonal NI 6014 (HP 6014) IgG1 kappa Biotin

产品编号 ABIN457888

发货至: 中国

Quick Overview for 小鼠 anti-人 IgG2 Antibody (Biotin) (ABIN457888)

抗原

IgG2

克隆类型

单克隆

标记

25
9
8
7
3
2
1
1
1
1
1

Biotin

应用范围

31
30
25
21
15
8
8
7
6
5
4
3
2
1

Western Blotting (WB), ELISA

克隆位点

NI 6014 (HP 6014)

亚型

IgG1 kappa

适用
22

13

9

7

7

1
人
宿主
21

11

11

10

6
小鼠

特异性

The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab portion of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Biotin-conjugated purified monoclonal mouse antibody to human IgG2, subclass specific

产品特性

Biotin/IgG protein molar ratio (B/P) approximately 4.7.

纯化方法

Purified

免疫原

Highly purified monoclonal IgG2 isolated from pooled human serum.
Mouse anti-Human IgG2 (Fd Region) antibody
WB, ELISA, IHC, ICC Monoclonal HP6014 IgG1 unconjugated

Mouse anti-Human IgG2 (Fc Region) antibody
ELISA Monoclonal HP6002 IgG1 unconjugated

Mouse anti-Human IgG2 (Fc Region) antibody (PE)
ELISA Monoclonal HP6002 IgG1 PE

Mouse anti-Human IgG2 (Fd Region) antibody (Biotin)
WB, ELISA, IHC, ICC Monoclonal HP6014 IgG1 Biotin

Mouse anti-Human IgG2 (Fc Region) antibody (Biotin)
ELISA Monoclonal 31-7-4 IgG1 Biotin

Mouse anti-Human IgG2 (Fc Region) antibody (FITC)
ELISA, FLISA Monoclonal 31-7-4 IgG1 FITC

Mouse anti-Human IgG2 (Fc Region) antibody (HRP)
WB, ELISA, IHC Monoclonal 31-7-4 IgG1 HRP

Mouse anti-Human IgG2 (Fc Region) antibody (Alkaline Phosphatase (AP))
WB, ELISA, IHC Monoclonal 31-7-4 IgG1 Alkaline Phosphatase (AP)

Mouse anti-Human IgG2 (Fd Region) antibody (HRP)
WB, ELISA, IHC Monoclonal HP6014 IgG1 HRP

Mouse anti-Human IgG2 antibody
WB, ELISA Monoclonal IGG-002 IgG1 unconjugated

应用备注

To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 250, in ELISA from 1:100 upwards, in Western blotting from 1:500 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

浓度

0.4 mg/mL

缓冲液

Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH 7.2).

储存液

Without preservative

注意事项

Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

储存条件

4 °C/-20 °C

储存方法

The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
抗原

IgG2

物质类

Antibody

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