小鼠 anti-人 IgG (Fc Region) Antibody (HRP)
Quick Overview for 小鼠 anti-人 IgG (Fc Region) Antibody (HRP) (ABIN457883)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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抗原表位
- Fc Region
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适用
- 人
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宿主
- 小鼠
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特异性
- The reactivity of the this monoclonal antibody is restricted to isotype specific determinants on the Fc part of IgG. The antiserum reacts with the 4 subclasses of human IgG, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic immunoglobulins.
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产品特性
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Horseradish peroxidase conjugated purified monoclonal mouse antibody to human IgG, isotype specific
Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2). -
纯化方法
- Purified
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免疫原
- Highly purified polyclonal IgG isolated from human serum.
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Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC
Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgG (Heavy Chain) antibody (beta-Gal)ELISA, WB Polyclonal IgG beta-Gal
Goat anti-Human IgG (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)
Mouse anti-Human IgG (Fc Region) antibody (SPRD)ELISA, FACS Monoclonal H2 IgG2b SPRD
Mouse anti-Human IgG (Fc Region) antibody (Cy5)ELISA, FACS Monoclonal H2 IgG2b Cy5
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应用备注
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To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:500, in ELISA from 1:1000 upwards, in Western blotting from 1:2000 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).
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储存液
- Without preservative
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注意事项
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. -
储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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- IgG
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物质类
- Antibody
抗原
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