小鼠 anti-人 IgG2 Antibody (HRP)
Quick Overview for 小鼠 anti-人 IgG2 Antibody (HRP) (ABIN457837)
抗原
克隆类型
标记
应用范围
克隆位点
亚型
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适用
- 人
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宿主
- 小鼠
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特异性
- The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab portion of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. Peroxidase conjugated purified monoclonal mouse antibody to human IgG2, subclass specific
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产品特性
- Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
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纯化方法
- Purified
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免疫原
- Highly purified monoclonal IgG2 isolated from pooled human serum
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Mouse anti-Human IgG2 (Fd Region) antibody
WB, ELISA, IHC, ICC Monoclonal HP6014 IgG1 unconjugated
Mouse anti-Human IgG2 (Fc Region) antibodyELISA Monoclonal HP6002 IgG1 unconjugated
Mouse anti-Human IgG2 (Fc Region) antibody (PE)ELISA Monoclonal HP6002 IgG1 PE
Mouse anti-Human IgG2 (Fd Region) antibody (Biotin)WB, ELISA, IHC, ICC Monoclonal HP6014 IgG1 Biotin
Mouse anti-Human IgG2 (Fc Region) antibody (Biotin)ELISA Monoclonal 31-7-4 IgG1 Biotin
Mouse anti-Human IgG2 (Fc Region) antibody (FITC)ELISA, FLISA Monoclonal 31-7-4 IgG1 FITC
Mouse anti-Human IgG2 (Fd Region) antibody (FITC)ELISA, IHC, ICC Monoclonal HP6014 IgG1 FITC
Mouse anti-Human IgG2 (Fc Region) antibody (HRP)WB, ELISA, IHC Monoclonal 31-7-4 IgG1 HRP
Mouse anti-Human IgG2 (Fc Region) antibody (Alkaline Phosphatase (AP))WB, ELISA, IHC Monoclonal 31-7-4 IgG1 Alkaline Phosphatase (AP)
Mouse anti-Human IgG2 (Fd Region) antibody (PE)ELISA, IHC, ICC Monoclonal HP6014 IgG1 PE
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应用备注
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To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immuno-peroxidase staining of cytoplasmic IgG2, and immunoblotting.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:25 to 1:100, in ELISA from 1:250 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).
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储存液
- Without preservative
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注意事项
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. -
储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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- IgG2
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物质类
- Antibody
抗原
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