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小鼠 anti-人 IgG1 (Fc Region) Antibody (HRP)

ELISA, WB Monoclonal NI 132 (HP 6186) IgG1 kappa HRP
产品编号 ABIN457836
发货至: 中国
  • 抗原 See all IgG1 products
    IgG1
    抗原表位
    • 41
    • 25
    • 9
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
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    Fc Region
    适用
    • 95
    • 24
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    宿主
    • 60
    • 42
    • 39
    • 15
    • 10
    • 6
    • 1
    小鼠
    克隆类型
    • 92
    • 60
    单克隆
    标记
    • 46
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    • 1
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    HRP
    应用范围
    • 89
    • 59
    • 51
    • 45
    • 30
    • 19
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    ELISA, Western Blotting (WB)
    特异性
    The reactivity of the antiserum is restricted to the subclass specific determinant on the Fc portion of the IgG1 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Peroxidase conjugated purified monoclonal mouse antibody to human IgG1, subclass specific
    产品特性
    Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
    纯化方法
    Purified
    免疫原
    Highly purified monoclonal IgG1 isolated from pooled human serum.
    克隆位点
    NI 132 (HP 6186)
    亚型
    IgG1 kappa
  • 应用备注
    To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting.
    The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40, in ELISA from 1:125 to 1:8,000, in Western blotting from 1:250 to 1:10.000. These data should be interpreted as general recommendations only.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
    浓度
    0.4 mg/mL
    缓冲液
    Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Centrifuge product if not completely clear after standing at room temperature.
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    储存条件
    4 °C/-20 °C
    储存方法
    The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
  • 抗原
    IgG1
    Abstract
    IgG1 产品
    物质类
    Antibody
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