小鼠 anti-人 Bence-Jones Protein kappa (Chain kappa), (Free & Bound) Antibody
ELISA, WB
Monoclonal
NI 285 and NI 250
IgG1 kappa
unconjugated
Quick Overview for 小鼠 anti-人 Bence-Jones Protein kappa (Chain kappa), (Free & Bound) Antibody (ABIN457818)
抗原
Bence-Jones Protein kappa
克隆类型
单克隆
应用范围
ELISA, Western Blotting (WB)
克隆位点
NI 285 and NI 250
亚型
IgG1 kappa
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抗原表位
- Chain kappa, Free & Bound
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适用
- 人
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宿主
- 小鼠
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产品特性
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The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immuno-peroxidase staining. Purified monoclonal mouse antibody to human Ig kappa chain, free and bound
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纯化方法
- Purified
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免疫原
- Highly purified Bence Jones kappa proteins isolated from pooled human urine.
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应用备注
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To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidase staining and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
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浓度
- 0.4 mg/mL
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缓冲液
- Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
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储存液
- Without preservative
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注意事项
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Bence-Jones Protein kappa
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别名
- Bence Jones kappa
抗原
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