山羊 anti-豚鼠 IgG2 (Subclass specific) Antibody (Biotin) - Preadsorbed
WB, ELISA, ICC, IHC (p), DB
Polyclonal
IgG
Biotin
(1 reference)
产品编号 ABIN457760
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 山羊 anti-豚鼠 IgG2 (Subclass specific) Antibody (Biotin) - Preadsorbed (ABIN457760)
抗原
IgG2
克隆类型
多克隆
标记
Biotin
应用范围
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)
亚型
IgG
-
-
抗原表位
- Subclass specific
-
适用
- 豚鼠
-
宿主
- 山羊
-
原理
- Goat anti Guinea Pig IgG2 (subclass specific), conjugated with Biotin
-
特异性
- Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Guinea Pig IgG2, subclass specific
-
交叉反应 （详细）
- This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this antiSerum has not been tested in detail.
-
产品特性
- The reactivity of the antiserum is directed to the subclass IgG2. It does not react with IgG1, IgG/Fab fragments, IgM and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
-
纯化方法
- Purified
-
免疫原
- Purified IgG2 isolated from guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
-
-
-
应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
-
缓冲液
- Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
-
-
-
: "Virus-specific immune memory at peripheral sites of herpes simplex virus type 2 (HSV-2) infection in guinea pigs." in: PLoS ONE, Vol. 9, Issue 12, pp. e114652, (2016) (PubMed).
-
-
- IgG2
-
物质类
- Antibody
抗原
-
You are here:
