兔 anti-Cow IgG (Heavy & Light Chain) Antibody (TRITC)
ELISA, IF, ICC, IHC (fro)
Polyclonal
TRITC
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- TRITC
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应用范围
- ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Bovine IgG, heavy and light chains.
- 交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion with the following results: Cat + Chicken - Dog + Duck - Goat ++ Guinea Pig + Hamster + Horse ++ Human ++ Mouse + Monkey + pigeon - Rat + Sheep ++ Swine + Turkey - A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
- 产品特性
- The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in bovine serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two Nordic immunoconjugates with different labels but originating from the same host animal species are used for direct two-color staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
- 纯化方法
- Purified
- 免疫原
- Purified normal IgG isolated from pooled bovine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Rabbit anti-Cow IgG (Fc Region) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated
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- 应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- It is reconstituted by adding 2 mL sterile distilled water.TRITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added It is reconstituted by adding 2 mL sterile distilled water.
- 缓冲液
- TRITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added
- 储存液
- Without preservative
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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