Applications: Quality tested applications include - ELISA , FLISA FC ,
Other referenced applications include - ELISPOT , IHC-FS , IHC-PS , ICC , WB , Sep , Depletion
Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
0.5 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Do not freeze! Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
储存条件
4 °C
储存方法
Store at 2-8°C
Sundaravinayagam, Rahjouei, Andreani, Tupiņa, Balasubramanian, Saha, Delgado-Benito, Coralluzzo, Daumke, Di Virgilio: "53BP1 Supports Immunoglobulin Class Switch Recombination Independently of Its DNA Double-Strand Break End Protection Function." in: Cell reports, Vol. 28, Issue 6, pp. 1389-1399.e6, (2019) (PubMed).
Delgado-Benito, Rosen, Wang, Gazumyan, Pai, Oliveira, Sundaravinayagam, Zhang, Andreani, Keller, Kieffer-Kwon, Pękowska, Jung, Driesner, Subbotin, Casellas, Chait, Nussenzweig, Di Virgilio: "The Chromatin Reader ZMYND8 Regulates Igh Enhancers to Promote Immunoglobulin Class Switch Recombination." in: Molecular cell, Vol. 72, Issue 4, pp. 636-649.e8, (2019) (PubMed).
Li, Irrazabal, So, Berru, Du, Lam, Ling, Gommerman, Pan-Hammarström, Martin: "The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining." in: Nature communications, Vol. 9, Issue 1, pp. 1006, (2018) (PubMed).