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山羊 anti-小鼠 IgG1 (Heavy Chain) Antibody (HRP)

ELISA, WB, IHC Polyclonal IgG HRP

Lane 1 - DEC Monoclonal AntibodyLane 2 - DEC-HER2 Monoclonal AntibodyLane 3 - Control Ig-HER2 Monoclonal AntibodyHER2 extracellular domain was cloned in frame into the COOH terminus of anti-DEC (DEC-HER2). (山羊 anti-小鼠 IgG1 (Heavy Chain) Antibody (HRP))

1 image

(2 references)

产品编号 ABIN376248

发货至: 中国

Datasheet as PDF

Quick Overview for 山羊 anti-小鼠 IgG1 (Heavy Chain) Antibody (HRP) (ABIN376248)

抗原

IgG1

克隆类型

多克隆

标记

HRP

应用范围

88
65
51
51
30
25
19
16
14
13
11
10
9
8
4
3
2
2
2

ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

亚型

IgG

抗原表位
40

25

9

6

4

3

3

2

2

1

1

1

1

1

1
Heavy Chain
适用
96

28

24

9

7

5

4

3

3

2

1
小鼠
宿主
59

45

43

15

10

6

1
山羊

特异性

Reacts with the heavy chain of mouse IgG1 as demonstrated by ELISA and flow cytometry.

产品特性

Source: Pooled antisera from goats hyperimmunized with mouse IgG 1 paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, Fluorescent ELISA, and/or flow cytometry to conform to characteristics of a standard reference reagent.

纯化方法

Affinity chromatography on mouse IgG1 covalently linked to agarose. Cross Absorption: Mouse IgM, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.
Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)

Rat anti-Mouse IgG1 antibody
ELISA, FACS Monoclonal SB77e IgG2b unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody
ELISA, WB, IHC, FACS Polyclonal IgG unconjugated

Rat anti-Mouse IgG1 antibody (HRP)
ELISA Monoclonal SB77E IgG2b HRP

Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC)
ELISA, IHC, FACS Polyclonal IgG FITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)
ELISA, WB, IHC, FACS Polyclonal IgG Biotin

Goat anti-Mouse IgG1 antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Cy5)
IHC, FACS Polyclonal IgG Cy5

Goat anti-Mouse IgG1 antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE

应用备注

Working Dilution:
ELISA: 1:4,000-1:8,000
Indirect immunostaining in conjunction with mouse IgG1 monoclonal antibodies.
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

限制

仅限研究用
状态

Liquid

注意事项

Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

储存条件

4 °C/-20 °C

储存方法

Store at 2-8 °C or long-term at -20 °C.
Margry, Kersemakers, Hoek, Arkesteijn, Wieland, van Eden, Broere: "Activated peritoneal cavity B-1a cells possess regulatory B cell properties." in: PLoS ONE, Vol. 9, Issue 2, pp. e88869, (2014) (PubMed).

Stegmann, Kamphuis, Meijerhof, Goud, de Haan, Wilschut: "Lipopeptide-adjuvanted respiratory syncytial virus virosomes: A safe and immunogenic non-replicating vaccine formulation." in: Vaccine, (2010) (PubMed).
抗原

IgG1

物质类

Antibody

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