小鼠 anti-人 IgE (Fc Region) Antibody
ELISA, WB, FACS, Func
Monoclonal
BSW17-NBS01
IgG1 kappa
unconjugated
2 images
(1 reference)
产品编号 ABIN363703
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 小鼠 anti-人 IgE (Fc Region) Antibody (ABIN363703)
抗原
IgE
克隆类型
单克隆
应用范围
ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
克隆位点
BSW17-NBS01
亚型
IgG1 kappa
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抗原表位
- Fc Region
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适用
- 人
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宿主
- 小鼠
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特异性
- Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
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产品特性
- IGHE, chromosome 14q32.33
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纯化方法
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
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纯度
- > 95 %, tested by analytical Gel Permeation Chromatography
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免疫原
- purified human myeloma IgE
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Mouse anti-Human IgE (Fc Region) antibody (Biotin)
ELISA Monoclonal HP6029 IgG2a Biotin
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应用备注
- When used for functional cellular assays (mast cell / basophil activation) at dilutions < 1:100, the Na-azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH=7.2, 0.1 % NaN3
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
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储存条件
- 4 °C
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: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).
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: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).
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- IgE
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背景
- The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
抗原
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