小鼠 anti-人 IgE (Fc Region) Antibody
ELISA, WB, FACS, Func
Monoclonal
Le27-NBS01
IgG1 kappa
unconjugated
2 images
(1 reference)Quick Overview for 小鼠 anti-人 IgE (Fc Region) Antibody (ABIN363693)
抗原
IgE
克隆类型
单克隆
应用范围
ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
克隆位点
Le27-NBS01
亚型
IgG1 kappa
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抗原表位
- Fc Region
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适用
- 人
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宿主
- 小鼠
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特异性
- Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes
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产品特性
- IGHE, chromosome 14q32.33
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纯化方法
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
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纯度
- > 95 %, tested by analytical Gel Permeation Chromatography
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免疫原
- purified human myeloma IgE
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Mouse anti-Human IgE (Fc Region) antibody (Biotin)
ELISA Monoclonal HP6029 IgG2a Biotin
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应用备注
- For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS, pH=7.2, 0.1 % NaN3
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
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储存条件
- 4 °C
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: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).
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: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).
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- IgE
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背景
- The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.
抗原
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