兔 anti-人 Ig (Chain kappa), (Light Chain) Antibody
IF, IHC (fro), IHC (p)
Polyclonal
IgG
unconjugated
Quick Overview for 兔 anti-人 Ig (Chain kappa), (Light Chain) Antibody (ABIN356485)
抗原
Ig
克隆类型
多克隆
应用范围
Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
亚型
IgG
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抗原表位
- Chain kappa, Light Chain
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适用
- 人, 猴
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宿主
- 兔
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纯化方法
- Purified
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免疫原
- Purified human Kappa light chain from human Bence Jones proteins.
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应用备注
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Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate pH 6.0 for10-20 minutes, followed by cooling at RT for 15-20 minutes). Recommended positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 2.0 mg/mL
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缓冲液
- PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative and 1 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Ig
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别名
- Immunoglobulin
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背景
- Human Kappa chain is a 25 kDa light chain present on all 5 immunoglobulin's (IgA,IgG, IgM, IgD, and IgE) and is also present in free form. Kappa and Lambda light chains consists of a variable region and a constant region and can be easily be differentiated by the antigenic properties of a constant region. The ratio of Kappa to Lambda chain is 70:30, most of them are bound to heavy chains of immunoglobulin.
抗原
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