兔 anti-人 Ig (Chain kappa) Antibody
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北京 101111
Quick Overview for 兔 anti-人 Ig (Chain kappa) Antibody (ABIN336978)
抗原
克隆类型
应用范围
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抗原表位
- Chain kappa
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适用
- 人, 猴
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宿主
- 兔
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特异性
- Molecular Weight: 25 kDa.
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免疫原
- Purified human Kappa light chain from human Bence Jones proteins
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应用备注
- IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:200 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate pH 6.0 for 10-20 minutes, followed by cooling at RT for 15-20 minutes). The optimum dilution should be determined by the individual lab. Positive Control: Human tonsil. Cellular Localization: Cytoplasmic.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- The antibody is supplied with 1% BSA in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- Ig
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别名
- Immunoglobulin Ig
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背景
- Human Kappa chain is a 25 kDa light chain present on all 5 immunoglobulin’s (IgA,IgG, IgM, IgD, and IgE) and is also present in free form. Kappa and Lambda light chains consists of a variable region and a constant region and can be easily be differentiated by the antigenic properties of a constant region. The ratio of Kappa to Lambda chain is 70:30, most of them are bound to heavy chains of immunoglobulin. This antibody is useful in identification of leukemias, plasmasacytomas and certain non-Hodgkin’s lymphomas. In normal human serum lambda chains are present 1.6-15.2 mg/ml. In multiple myeloma or B-cell malignancies these levels are increased. Higher amounts are also present in Bence Jones patient’s urine.
抗原
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