兔 anti-小鼠 IgG Antibody (Alkaline Phosphatase (AP))
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北京 101111
Quick Overview for 兔 anti-小鼠 IgG Antibody (Alkaline Phosphatase (AP)) (ABIN336833)
抗原
克隆类型
标记
应用范围
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适用
- 小鼠
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宿主
- 兔
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原理
- Rabbit anti-Mouse IgG (H&L) - Affinity Pure, ALP Conjugate, min x w/human serum proteins
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特异性
- Based on IEP, this antibody reacts with: • heavy (i³) chains on mouse IgG • light chains on all mouse immunoglobulins
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交叉反应 （详细）
- Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • human serum proteins
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纯化方法
- Affinity purified using solid phase Mouse IgG
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纯度
- Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0
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免疫原
- Purified Mouse IgG, whole molecule
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488)IHC, FACS, IF, ICC Polyclonal IgG DyLight 488
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488) - PreadsorbedIHC, FACS, WB, IF, ICC Polyclonal IgG DyLight 488
Donkey anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488) - PreadsorbedIHC, FACS, WB, IF, ICC Polyclonal IgG DyLight 488
Donkey anti-Mouse IgG (Heavy & Light Chain) antibody (DyLight 488)IHC, FACS, IF, ICC Polyclonal IgG DyLight 488
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应用备注
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This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:2,000 for ELISA/Western blot 1:20. 1:2,000 for Immunohistochemistry 1:50. 1:5,000 for Immunocytochemistry -
说明
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Country of Origin: Rabbit antiserum was obtained from animals of US origin and under the care of a registered veterinarian.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.5 mg/mL
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缓冲液
- 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
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- IgG
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物质类
- Antibody
抗原
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