山羊 anti-人 IgG Antibody (Alkaline Phosphatase (AP))
Quick Overview for 山羊 anti-人 IgG Antibody (Alkaline Phosphatase (AP)) (ABIN336567)
抗原
克隆类型
标记
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适用
- 人
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宿主
- 山羊
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原理
- Goat anti-Human IgG (H&L) - Affinity Pure, ALP Conjugate
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特异性
- Based on IEP, this antibody reacts with: • heavy (i³) chains on human IgG • light chains on all human immunoglobulins
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交叉反应 （详细）
- Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins
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纯化方法
- Affinity purified using solid phase Human IgG
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纯度
- Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0
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免疫原
- Purified Human IgG, whole molecule
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Mouse anti-Human IgG (Fc Region) antibody
Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP
Mouse anti-Human IgG (Fab Region) antibody (PE)FACS Monoclonal 4A11 IgG1 kappa PE
Rabbit anti-Human IgG (AA 167-185), (Internal Region) antibodyIHC (p) Monoclonal E20-V IgG unconjugated
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Donkey anti-Human IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgG (Heavy Chain) antibody (beta-Gal)ELISA, WB Polyclonal IgG beta-Gal
Goat anti-Human IgG (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)
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说明
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ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.5 mg/mL
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缓冲液
- 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
抗原
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