驴 anti-小鼠 IgG Antibody (HRP)
Quick Overview for 驴 anti-小鼠 IgG Antibody (HRP) (ABIN336465)
抗原
克隆类型
标记
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适用
- 小鼠
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宿主
- 驴
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原理
- Donkey anti-Mouse IgG (H&L) - Affinity Pure, HRP Conjugate
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特异性
- Based on IEP, this antibody reacts with: • heavy (i³) chains on mouse IgG • light chains on all mouse immunoglobulins
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交叉反应 （详细）
- Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins
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纯化方法
- Affinity purified using solid phase Mouse IgG
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纯度
- Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0
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免疫原
- Purified Mouse IgG, whole molecule
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM, DB Polyclonal IgG TRITC
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Cy5)IHC, FACS Polyclonal IgG Cy5
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE F(ab')2 fragment
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说明
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HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 1 % BSA & 0.1 % proclin 150, contains 0.1 % (v/v) Proclin 150
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50 % glycerol and store at -20 °C as a liquid.
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储存条件
- 4 °C
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储存方法
- Store freeze-dried powder at 2-8 °C.
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- IgG
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物质类
- Antibody
抗原
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