Our Local Distributor
北京 101111
Quick Overview for 小鼠 anti-人 IgM Antibody (ABIN3025946)
抗原
克隆类型
应用范围
克隆位点
亚型
-
-
适用
- 人
-
宿主
- 小鼠
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Heavy chain of human IgM was used as the immunogen for the Anti-IgM antibody.
-
-
Mouse anti-Human IgM antibody (FITC)
FACS Monoclonal CH2 IgG1 FITC
Mouse anti-Human IgM antibody (APC)FACS Monoclonal CH2 IgG1 APC
Mouse anti-Human IgM antibody (PE)FACS Monoclonal CH2 IgG1 PE
Mouse anti-Human IgM antibody (iFluor™647)GMP Grade FACS Monoclonal M-2E6 IgG1 iFluor™647
Mouse anti-Human IgM antibody (iFluor™700)GMP Grade FACS Monoclonal M-2E6 IgG1 iFluor™700
Mouse anti-Human IgM antibody (mFluor™450)GMP Grade FACS Monoclonal M-2E6 IgG1 mFluor™450
Mouse anti-Human IgM antibody (PE-Cy7)GMP Grade FACS Monoclonal M-2E6 IgG1 PE-Cy7
Goat anti-Human IgM antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human IgM (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Human IgM (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
-
-
应用备注
-
Optimal dilution of the Anti-IgM antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
-
-
-
浓度
- 0.2 mg/mL
-
缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store the Anti-IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
-
- IgM
-
物质类
- Antibody
-
背景
- Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
抗原
-
