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小鼠 anti-人 IgG (Fc Region) Antibody

ELISA, WB, FACS, ICC Monoclonal EM-07 IgG1 unconjugated

IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B). (小鼠 anti-人 IgG (Fc Region) Antibody)

Flow cytometry surface staining pattern of human peripheral whole blood using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/mL, GAM APC). (小鼠 anti-人 IgG (Fc Region) Antibody)

Separation of human monocytes (red-filled) from CD3 negative IgG (Fc) positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/mL, GAM APC). (小鼠 anti-人 IgG (Fc Region) Antibody)

Flow cytometry multicolor surface staining of human peripheral whole blood stained using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood). (小鼠 anti-人 IgG (Fc Region) Antibody)

Flow cytometry surface staining pattern of human peripheral whole blood using anti-human IgG (Fc) (EM-07) purified antibody (concentration in sample 1 μg/mL, GAM APC). (小鼠 anti-人 IgG (Fc Region) Antibody)

5 images

产品编号 ABIN302085

发货至: 中国

Quick Overview for 小鼠 anti-人 IgG (Fc Region) Antibody (ABIN302085)

抗原

IgG

克隆类型

单克隆

应用范围

ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

克隆位点

EM-07

亚型

IgG1

抗原表位
2243

315

286

149

130

67

52

29

28

28

21

5

5

2

1

1

1

1

1

1

1
Fc Region
适用
610

547

529

395

344

231

186

154

130

124

109

86

84

82

82

62

47

28

27

16

15

10

5

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
人
宿主
1479

1364

523

206

98

83

38

29

21

20

16

11

11
小鼠

原理

Anti-Hu IgG (Fc) Purified

特异性

The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.

交叉反应（详细）

Human, Other not determined. The antibody EM-07 does not crossreact with IgM.

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Fusion protein of human IgG Fc fragment.
Mouse anti-Human IgG (Fab Region) antibody (PE)
FACS Monoclonal 4A11 IgG1 kappa PE

Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC

Donkey anti-Human IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - Preadsorbed
FACS, FLISA, FM Polyclonal IgG Texas Red (TR)

Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Human IgG (Heavy & Light Chain) antibody
ELISA, IHC, WB Polyclonal IgG unconjugated

Goat anti-Human IgG (Heavy Chain) antibody (beta-Gal)
ELISA, WB Polyclonal IgG beta-Gal

Goat anti-Human IgG (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Baboon IgG (Heavy & Light Chain) antibody
ELISA, ID Polyclonal IgG unconjugated

Rabbit anti-Human IgG (Heavy & Light Chain) antibody (DyLight 594)
FACS, IF DyLight 594

Goat anti-Baboon IgG (Heavy & Light Chain) antibody (FITC)
FACS, IF, ICC, IHC (fro) Polyclonal IgG FITC

应用备注

Flow cytometry: Recommended dilution: 1-4 μg/mL.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
抗原

IgG

物质类

Antibody

背景

Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.,Immunoglobulin G Fc fragment

分子量

150 kDa

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