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小鼠 anti-人 IgG (Fc Region) Antibody (HRP)

ELISA, WB Monoclonal EM-07 IgG1 HRP

IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B). (小鼠 anti-人 IgG (Fc Region) Antibody (HRP))

ELISA detection of IgG in human serum using HRP conjugated EM-07 antibody against IgG Fc fragment. (小鼠 anti-人 IgG (Fc Region) Antibody (HRP))

2 images

产品编号 ABIN302063

发货至: 中国

Datasheet as PDF

Quick Overview for 小鼠 anti-人 IgG (Fc Region) Antibody (HRP) (ABIN302063)

抗原

IgG

克隆类型

单克隆

标记

HRP

应用范围

ELISA, Western Blotting (WB)

克隆位点

EM-07

亚型

IgG1

抗原表位
2397

516

417

146

130

128

66

29

28

28

21

9

5

5

2

2

2

2

2

1

1

1
Fc Region
适用
751

703

607

468

410

244

213

176

146

141

124

110

107

101

82

62

47

28

27

16

15

10

8

5

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
人
宿主
1773

1583

567

281

122

112

41

32

22

21

15

11

11
小鼠

原理

Anti-Hu IgG (Fc) HRP

特异性

The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.

交叉反应（详细）

Human, Other not determined

纯化方法

Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.

免疫原

Fusion protein of human IgG Fc fragment.
Mouse anti-Human IgG (Fc Region) antibody
ELISA, WB, FACS, ICC Monoclonal EM-07 IgG1 unconjugated

Mouse anti-Human IgG (Fc Region) antibody
Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody unconjugated

Mouse anti-Human IgG (Fc Region) antibody (HRP)
ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP

Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG1 kappa unconjugated

Mouse anti-Human IgG (Fc Region) antibody (HRP)
Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP

Goat anti-Human IgG antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC

Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Human IgG (Heavy & Light Chain) antibody
ELISA, IHC, WB Polyclonal IgG unconjugated

Goat anti-Human IgG antibody
ELISA Polyclonal IgG unconjugated Fab fragment

应用备注

Western blotting and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.

限制

仅限研究用
状态

Liquid

浓度

0.2 mg/mL

缓冲液

Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01 % thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
抗原

IgG

物质类

Antibody

背景

Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.,Immunoglobulin G Fc fragment

分子量

150 kDa

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