(1 reference)Quick Overview for 小鼠 anti-小鼠 IgMb Antibody (Biotin) (ABIN2689713)
抗原
IgMb
克隆类型
单克隆
标记
Biotin
应用范围
Flow Cytometry (FACS), ELISA
克隆位点
AF6
亚型
IgG1 kappa
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适用
- 小鼠
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宿主
- 小鼠
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品牌
- BD Pharmingen™
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产品特性
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The AF6-78 antibody reacts specifically with mouse IgM of Igh-C[b] and related haplotypes (e.g., C57BL/6, C57BL/10, SJL, AKR, NZB). It does not react with IgM of Igh-C[a] or related haplotypes (e.g., BALB/c, C58, CBA, C3H/Bi, C3H/He, DBA/1, DBA/2, PL, RIII). Cross-reaction with IgM of Igh-C[e] haplotype (e.g., A/J) has been observed. AF6-78 antibody does not react with free μ heavy chain in vitro or in the cytoplasm of pre-B lymphocytes, which lack Ig light chain. It has not been shown to stimulate B-cell proliferation.
BD Pharmingen™ Biotin Mouse Anti-Mouse IgM[b] - Biotin - Clone AF6-78 - Isotype Mouse IgG1, κ - Reactivity Ms - 0.5 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- C57BL/10 mouse splenocytes
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 2, pp. 787-95, (1984) (PubMed).
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: "Allotypic specificities of murine IgD and IgM recognized by monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 2, pp. 787-95, (1984) (PubMed).
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- IgMb
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别名
- IgM[b]
抗原
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