小鼠 anti-小鼠 IgG2ab, Subclass Specific Antibody (Biotin)
ELISA, FACS
Monoclonal
5-7
IgG3 kappa
Biotin
(4 references)-
- 抗原 See all IgG2ab products
- IgG2ab (IgG2ab, Subclass Specific)
- 适用
- 小鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- Biotin
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应用范围
- ELISA, Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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This antibody reacts specifically with mouse IgG2a of Igh-C [b] haplotype (e.g., C57BL/6, C57BL/10, NOD, SJL). It has been reported not to react with IgG2a of the a, c, d, e, f, g, h, j, n, o, or p haplotypes (e.g., A, AKR, BALB/c, C3H/He, C58, CBA, DBA/1, DBA/2, NZB, SWR). Molecular genetic analyses suggest that the Igh-1 [b] allele, which encodes IgG2a [b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.
BD Pharmingen™ Biotin Mouse Anti-Mouse IgG2a [b] - Biotin - Clone 5.7 - Isotype Mouse IgG3, κ - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Not Reported
- 克隆位点
- 5-7
- 亚型
- IgG3 kappa
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
-
-
: "The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype." in: Immunogenetics, Vol. 46, Issue 2, pp. 167-8, (1997) (PubMed).
: "Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes." in: The EMBO journal, Vol. 8, Issue 11, pp. 3245-51, (1989) (PubMed).
: "Genetic characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies." in: Immunogenetics, Vol. 18, Issue 4, pp. 311-21, (1983) (PubMed).
: "Structural characterization of mouse immunoglobulin allotypic determinants (allotopes) defined by monoclonal antibodies." in: Immunogenetics, Vol. 18, Issue 4, pp. 323-34, (1983) (PubMed).
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: "The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype." in: Immunogenetics, Vol. 46, Issue 2, pp. 167-8, (1997) (PubMed).
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- 抗原
- IgG2ab (IgG2ab, Subclass Specific)
- 别名
- IgG2a [b] (IgG2ab 产品)
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