山羊 anti-小鼠 Ig Antibody (Biotin) - Preadsorbed
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Quick Overview for 山羊 anti-小鼠 Ig Antibody (Biotin) - Preadsorbed (ABIN2689598)
抗原
克隆类型
标记
应用范围
亚型
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适用
- 小鼠
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宿主
- 山羊
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产品特性
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This polyclonal antibody reacts with whole molecule mouse IgG (heavy and light chains) and may also react with the light chains of other mouse immunoglobulins. This antibody has been reported to be reactive in ELISA with mouse IgG, IgM, and IgA. In addition, low level cross-reactivity with rat and hamseter Ig has been reported. Investigators should note that reactivity with some mouse IgM antibodies can be weak. Minimal cross-reaction to rat, human, bovine, horse and rabbit non-immunoglobulin serum proteins are expected. Note: This reagent may have considerable cross-reactivity on human peripheral blood leukocytes, contributing to undesired background signal, when used with the flow cytometric application. As a result, this product is not recommended for immunofluorescent staining and flow cytometric analysis of human cells. For this application, investigators may wish to consider FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988) as an alternative. For immunohistochemical staining, Biotin Goat Anti-Mouse Ig (Cat. No. 550337) is recommended.
BD Pharmingen™ Biotin Goat Anti-Mouse Ig (Multiple Adsorption) - Biotin - Clone Polyclonal - Isotype Goat Ig - Reactivity Ms - 0.5 mg -
纯化方法
- Preadsorption: Negative adsorption
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Goat anti-Mouse Ig antibody (PE) - Preadsorbed
FACS, FLISA Polyclonal IgG PE
Goat anti-Mouse Ig (Chain kappa) antibodyELISA, FACS, WB, IHC Polyclonal IgG unconjugated
Goat anti-Mouse Ig antibody - PreadsorbedELISA, FACS, WB, IHC (fro), IHC (p), ICC, ELISpot Polyclonal IgG unconjugated
Goat anti-Mouse Ig antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), ICC, ELISpot Polyclonal IgG HRP
Goat anti-Mouse Ig antibody (SPRD) - PreadsorbedELISA, FACS Polyclonal IgG SPRD F(ab')2 fragment
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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- Ig
抗原
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