驴 anti-绵羊 IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed
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- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Heavy & Light Chain
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适用
- 绵羊
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宿主
- 驴
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克隆类型
- 多克隆
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标记
- PE
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应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Sheep IgG and Sheep Serum.
- 交叉反应
- 绵羊
- 产品特性
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Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated antibody generated in donkey detects specifically sheep IgG. This secondary phycoerythrin conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.
Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated antibody generated in donkey detects specifically sheep IgG. This secondary phycoerythrin conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis. - 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Anti-Sheep IgG was produced by repeated immunization with sheep IgG whole molecule in donkey.
Immunogen Type: Native Protein
- 亚型
- IgG
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Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (FITC) - Preadsorbed
FACS, FLISA, FM Polyclonal IgG FITC
Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC Fab fragment
Rabbit anti-Sheep IgG (F(ab')2 Region) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFM Polyclonal IgG TRITC F(ab')2 fragment
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- 应用备注
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Application Note: Antibody Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Optimal titers for other applications should be determined by the researcher.
Flow Cytometry Dilution: 1:100 - 1:250
IF Microscopy Dilution: 1:100 - 1:250
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 0.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- RT,4 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Donkey F(ab')2 Anti-Sheep IgG Antibody Phycoerythrin Conjugation, Donkey F(ab')2 Anti-Sheep IgG PE Conjugated Antibody
Background: Anti-Sheep F(ab')2 IgG (H&L) phycoerythrin conjugated antibody generated in donkey detects specifically sheep IgG. This secondary phycoerythrin conjugated antibody anti-Sheep is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.
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